Ontology highlight
ABSTRACT:
SUBMITTER: Jimenez AI
PROVIDER: S-EPMC3416946 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Insect biochemistry and molecular biology 20120620 9
Bacillus thuringiensis subs. israelensis produces at least three Cry toxins (Cry4Aa, Cry4Ba, and Cry11Aa) that are active against Aedes aegypti larvae. Previous work characterized a GPI-anchored alkaline phosphatase (ALP1) as a Cry11Aa binding molecule from the gut of A. aegypti larvae. We show here that Cry4Ba binds ALP1, and that the binding and toxicity of Cry4Ba mutants located in loop 2 of domain II is correlated. Also, we analyzed the contribution of ALP1 toward the toxicity of Cry4Ba and ...[more]