Recombinant human decorin suppresses liver HepG2 carcinoma cells by p21 upregulation.

ABSTRACT:

Background

Decorin is a multifunctional molecule of the extracellular matrix and impedes different kinds of tumor cell growth, but the role and molecular mechanism by which decorin inhibits HepG2 cells is not fully understood. Our objective was to construct recombinant human decorin (pcDNA3.1-DCN) and to explore the mechanism by which it inhibits HepG2 cells.

Methods

This experiment was divided into three groups, ie, a control group, an empty vector group, and a pcDNA3.1-DCN group. pcDNA3.1-DCN was constructed using recombinant DNA technology, and the vector for pcDNA3.1-DCN and pcDNA3.1 was then transfected into HepG2 cells using Lipofectamine 2000.

Results

Compared with cells in the control group and in the empty vector group, growth of cells in the pcDNA3.1-DCN gro

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3422087 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature