Dataset Information
Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar.
Ontology highlight
ABSTRACT: Background
A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.Methods
A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.Results
With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified.Conclusion
The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.
SUBMITTER: Nazemalhosseini-Mojarad E
PROVIDER: S-EPMC3488828 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar.
Iranian journal of parasitology 20120101 1
<h4>Background</h4>A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.<h4>Methods</h4>A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR ...[more]
