Sequence Diversity in tRNA Gene Locus A-L among Iranian Isolates of Entamoeba dispar.

ABSTRACT:

Background

A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.

Methods

A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.

Results

With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locu

SUBMITTER: Nazemalhosseini-Mojarad E 

PROVIDER: S-EPMC3488828 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature