In vivo analysis of interactions between GFP-labeled microfilaments and plastid stromules.

ABSTRACT:

Background

Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled microfilaments and plastids.

Results

We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe microfilaments and their relationship to stromules in vivo. Microfilament

SUBMITTER: Kwok EY 

PROVIDER: S-EPMC356911 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature