Microsatellite markers for an endemic Atlantic Forest tree, Manilkara multifida (Sapotaceae).

ABSTRACT: Manilkara multifida is a tropical tree that is endemic to the Atlantic forests of southern Bahia, Brazil. Currently, populations of this species are restricted to fragmented landscapes that are susceptible to anthropogenic disturbances. Considering this issue, and that there is no genetic information available for this endangered species, we developed microsatellite markers for M. multifida to provide resources for future conservation genetics studies. Using an enriched genomic library, we isolated eight polymorphic microsatellite loci and optimized the amplification conditions for M. multifida. For each locus, we estimated the number of alleles, H E and H O, paternity exclusion Q, individual identity I and fixation index F, and examined the presence of null alleles. The mean number of all

SUBMITTER: Moraes RC 

PROVIDER: S-EPMC3595987 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature