Hypercholesterolemia potentiates aortic endothelial response to inhaled diesel exhaust.

ABSTRACT:

Background

Inhalation of diesel exhaust induces vascular effects including impaired endothelial function and increased atherosclerosis.

Objective

To examine the in vivo effects of subchronic diesel exhaust exposure on endothelial cell transcriptional responses in the presence of hypercholesterolemia.

Methods

ApoE (-/-) and ApoE (+/+) mice inhaled diesel exhaust diluted to particulate matter levels of 300 or 1000 μg/m³ vs. filtered air. After 30 days, endothelial cells were harvested from dispersed aortic cells by fluorescent-activated cell sorting (FACS). Relative mRNA abundance was evaluated by microarray analysis to measure strain-specific transcriptional responses in mice exposed to dilute diesel exhaust vs. filtered air.

Results

Forty-nine transcripts were

SUBMITTER: Maresh JG 

PROVIDER: S-EPMC3606052 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature