Ontology highlight
ABSTRACT:
SUBMITTER: Zhou ZX
PROVIDER: S-EPMC3613587 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Genome research 20121217 4
Spontaneous DNA damage may occur nonrandomly in the genome, especially when genome maintenance mechanisms are undermined. We developed single-strand DNA (ssDNA)-associated protein immunoprecipitation followed by sequencing (SPI-seq) to map genomic hotspots of DNA damage. We demonstrated this method with Rad52, a homologous recombination repair protein, which binds to ssDNA formed at DNA lesions. SPI-seq faithfully detected, in fission yeast, Rad52 enrichment at artificially induced double-strand ...[more]