Suppression of Human Liver Cancer Cell Migration and Invasion via the GABAA Receptor.

ABSTRACT:

Objective

To investigate the roles of the γ-aminobutyric acid (GABA) in the metastasis of hepatocellular carcinoma (HCC) and to explore the potential of a novel therapeutic approach for the treatment of HCC.

Methods

The expression levels of GABA receptor subunit genes in various HCC cell lines and patients' tissues were detected by quantitative real-time polymerase chain reaction and Western blot analysis. Transwell cell migration and invasion assays were carried out for functional analysis. The effects of GABA on liver cancer cell cytoskeletal were determined by immunofluorescence staining. And the effects of GABA on HCC metastasis in nude mice were evaluated using an in vivo orthotopic model of liver cancer.

Results

The mRNA level of GABA receptor subunits varied be

SUBMITTER: Chen ZA 

PROVIDER: S-EPMC3643652 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature