Crystallization of recombinant green mamba ρ-Da1a toxin during a lyophilization procedure and its structure determination.

ABSTRACT: ρ-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled α(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 Å, and diffracted to 1.95 Å resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins.

SUBMITTER: Maiga A 

PROVIDER: S-EPMC3668600 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature