Dataset Information
A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.
Ontology highlight
ABSTRACT: Background
Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.Objectives
We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.Methods
We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results from the C. elegans assay with the comprehensive mammalian in vivo end point data from the ToxRef database.Results
The assay was highly predictive of mammalian reproductive toxicities, with a 69% maximum balanced accuracy. We confirmed the effect of select compounds on germline integrity by monitoring germline apoptosis and meiotic progression.Conclusions
This C. elegans assay provides a comprehensive strategy for assessing environmental effects on germline function.
SUBMITTER: Allard P
PROVIDER: S-EPMC3672921 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Publications
A C. elegans screening platform for the rapid assessment of chemical disruption of germline function.
Environmental health perspectives 20130419 6
<h4>Background</h4>Despite the developmental impact of chromosome segregation errors, we lack the tools to assess environmental effects on the integrity of the germline in animals.<h4>Objectives</h4>We developed an assay in Caenorhabditis elegans that fluorescently marks aneuploid embryos after chemical exposure.<h4>Methods</h4>We qualified the predictive value of the assay against chemotherapeutic agents as well as environmental compounds from the ToxCast Phase I library by comparing results fr ...[more]
