Comparison of the LTQ-Orbitrap Velos and the Q-Exactive for proteomic analysis of 1-1000 ng RAW 264.7 cell lysate digests.

ABSTRACT:

Rationale

There is interest in extending bottom-up proteomics to the smallest possible sample size. We investigated the performance of two modern mass spectrometers for the analysis of samples ranging from 1 ng to 1 µg of RAW 264.7 cell lysate digests.

Methods

An ultra-performance liquid chromatography (UPLC) system coupled with either an LTQ-Orbitrap Velos or a Q-Exactive mass spectrometer was used for peptide separation and identification.

Results

For 1-1000 ng RAW 264.7 cell lysate digests, the Q-Exactive generated 10-83% more protein groups and 11-109% more peptides than the LTQ-Orbitrap Velos (higher-energy collisional dissociation, HCD) with MASCOT database searching, due to its faster scan rate and higher resolution. In addition, HCD and collision-induced disso

SUBMITTER: Sun L 

PROVIDER: S-EPMC3673017 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature