RRNA:mRNA pairing alters the length and the symmetry of mRNA-protected fragments in ribosome profiling experiments.

ABSTRACT:

Motivation

Ribosome profiling is a new technique that allows monitoring locations of translating ribosomes on mRNA at a whole transcriptome level. A recent ribosome profiling study demonstrated that internal Shine-Dalgarno (SD) sequences have a major global effect on translation rates in bacteria: ribosomes pause at SD sites in mRNA. Therefore, it is important to understand how SD sites effect mRNA movement through the ribosome and generation of ribosome footprints.

Results

Here, we provide evidence that in addition to pausing effect, internal SD sequences induce a caterpillar-like movement of mRNA through the ribosome cavity. Once an SD site binds to the ribosome, it remains attached to it while the ribosome decodes a few subsequent codons. This leads to asymmetric progress

SUBMITTER: O'Connor PB 

PROVIDER: S-EPMC3673220 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature