Multiplexed Illumina sequencing libraries from picogram quantities of DNA.

ABSTRACT:

Background

High throughput sequencing is frequently used to discover the location of regulatory interactions on chromatin. However, techniques that enrich DNA where regulatory activity takes place, such as chromatin immunoprecipitation (ChIP), often yield less DNA than optimal for sequencing library preparation. Existing protocols for picogram-scale libraries require concomitant fragmentation of DNA, pre-amplification, or long overnight steps.

Results

We report a simple and fast library construction method that produces libraries from sub-nanogram quantities of DNA. This protocol yields conventional libraries with barcodes suitable for multiplexed sample analysis on the Illumina platform. We demonstrate the utility of this method by constructing a ChIP-seq library from 100 p

SUBMITTER: Bowman SK 

PROVIDER: S-EPMC3711846 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature