The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.

ABSTRACT: Connexins are the structural subunits of gap junctions and act as protein platforms for signaling complexes. Little is known about tissue-specific connexin signaling nexuses, given significant challenges associated with affinity-purifying endogenous channel complexes to the level required for interaction analyses. Here, we used multiple subcellular fractionation techniques to isolate connexin32-enriched membrane microdomains from murine liver. We show, for the first time, that connexin32 localizes to both the plasma membrane and inner mitochondrial membrane of hepatocytes. Using a combination of immunoprecipitation-high throughput mass spectrometry, reciprocal co-IP, and subcellular fractionation methodologies, we report a novel interactome validated using null mutant controls. Eighteen co

SUBMITTER: Fowler SL 

PROVIDER: S-EPMC3714164 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature