Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter.

ABSTRACT:

Introduction

The molecular links between shock-response and adaptation remain poorly understood, particularly for extremophiles. This has hindered rational engineering of solvent tolerance and correlated traits (e.g., productivity) in extremophiles. To untangle such molecular links, here we established a model that tracked the microevolution from shock to adaptation in thermophilic bacteria.

Method

Temporal dynamics of genomes and transcriptomes was tracked for Thermoanaerobacter sp. X514 which under increasing exogenous ethanol evolved from ethanol-sensitive wild-type (Strain X) to tolerance of 2%- (XI) and eventually 6%-ethanol (XII). Based on the reconstructed transcriptional network underlying stress tolerance, genetic engineering was employed to improve ethanol toleranc

SUBMITTER: Lin L 

PROVIDER: S-EPMC3751872 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature