Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.
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ABSTRACT: Objectives
To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root.Materials and methods
The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data.Results
The active fraction of S. tetragonum showed presence of anti-diabetes mellitus activity in type-2 diabetic rats. It did not significantly influence insulin release from cultured islets. Two active principles (active at 2 mg/kg dose) were isolated and characterized with spectral data. One of them was identified as an iridoid type glycoside and the other one was a lapachol like compound (derivative of naphthoquinone).Conclusions
Two active principles from the anti-diabetes fraction of S. tetragonum root were isolated and identified as an iridoid glycoside and a naphthoquinone derivative.
SUBMITTER: Bino Kingsley R
PROVIDER: S-EPMC3758101 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Anti-diabetic activity of active fractions of Stereospermum tetragonum DC and isolation of active principles.
Bino Kingsley Renjit R Mishra Manisha M Brindha Pemaiah P Subramoniam Appian A
Journal of young pharmacists : JYP 20130320 1
<h4>Objectives</h4>To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root.<h4>Materials and methods</h4>The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data.<h4>Results</h4>The active fraction of S. tetragonu ...[more]
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