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Detection of enterotoxin genes of Staphylococcus SP isolated from nasal cavities and hands of food handlers.


ABSTRACT: Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.

SUBMITTER: Rall VL 

PROVIDER: S-EPMC3768627 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Detection of enterotoxin genes of Staphylococcus SP isolated from nasal cavities and hands of food handlers.

Rall V L M VL   Sforcin J M JM   Augustini V C M VC   Watanabe M T MT   Fernandes A A   Rall R R   Silva M G MG   Araújo J P JP  

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20100101 1


Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotox  ...[more]

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