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Diversity of endophytic bacteria in medicinally important Nepenthes species.

ABSTRACT: BACKGROUND:Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp. OBJECTIVE:The objective of this study was to isolate and to identify EB associated with Nepenthes spp. MATERIALS AND METHODS:Surface-sterilized leaf and stem tissues from nine Nepenthes spp. collected from Peninsular Malaysia were used to isolate EB. Isolates were identified using the polymerase chain reaction-amplified 16S ribosomal DNA (rDNA) sequence similarity based method. RESULTS:Cultivable, 96 isolates were analyzed; and the 16S rDNA sequences analysis suggest that diverse bacterial species are associated with Nepenthes spp. Majority (55.2%) of the isolates were from Bacillus genus, and Bacillus cereus was the most dominant (14.6%) among isolates. CONCLUSION:Nepenthes spp. do harbor a wide array of cultivable endophytic bacteria.

SUBMITTER: Bhore SJ

PROVIDER: S-EPMC3783794 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Diversity of endophytic bacteria in medicinally important Nepenthes species.

Bhore Subhash J SJ Komathi Vijayan V Kandasamy Kodi I KI

Journal of natural science, biology, and medicine 20130701 2

<h4>Background</h4>Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp.<h4>Objective</h4>The objective of this study was to isolate and to identify EB associated with Nepenthes spp.<h4>Materials and methods</h4>Surface-sterilized leaf and stem tissues from nine Nepenthes spp. collected from Peninsular Malaysia were used to isolate EB. Isolates were identified using ...[more]

PMID: 24082746

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