Dexamethasone induces cross-linked actin networks in trabecular meshwork cells through noncanonical wnt signaling.

ABSTRACT:

Purpose

Dexamethasone (DEX) regulates aqueous humor outflow by inducing a reorganization of the cytoskeleton to form cross-linked actin networks (CLANs) in trabecular meshwork (TM) cells. Rho-associated protein kinase (ROCK) has been demonstrated to have an important role in this process, but the upstream components leading to its activation remain elusive. The purpose of the study is to demonstrate that noncanonical Wnt signaling mediates the DEX-induced CLAN formation in TM cells.

Methods

The TM cells were treated with 100 nM DEX in low serum medium for over 7 days. The medium was changed every 3 days. The cells were harvested and subjected to molecular analysis for the expression of Wnt ligands. Stress fiber structures were revealed by Phalloidin staining. Lentivirus-base

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC3790389 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature