HTRF analysis of soluble huntingtin in PHAROS PBMCs.

ABSTRACT:

Objective

We measured the levels of mutant huntingtin (mtHtt) and total huntingtin (tHtt) in blood leukocytes from Prospective Huntington At-Risk Observational Study (PHAROS) subjects at 50% risk of carrying the Huntington disease mutation using a homogeneous time-resolved fluorescence (HTRF) assay to assess its potential as a biomarker.

Methods

Peripheral blood mononuclear cells from consenting PHAROS subjects were analyzed by HTRF using antibodies that simultaneously measured mtHtt and tHtt. mtHtt levels were normalized to tHtt, double-stranded DNA, or protein and analyzed according to cytosine-adenine-guanine repeat length (CAGn), demographics, predicted time to clinical onset or known time since clinical onset, and available clinical measures.

Results

From 363 ass

SUBMITTER: Moscovitch-Lopatin M 

PROVIDER: S-EPMC3795598 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature