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Linking genotype to phenotype on beads: high throughput selection of peptides with biological function.

ABSTRACT: Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro through blocking of protein:protein interaction with the co-receptor CCR5. The bead display approach has many potential applications for probing biological systems and for drug lead development.

SUBMITTER: Huang LC

PROVIDER: S-EPMC3805977 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Linking genotype to phenotype on beads: high throughput selection of peptides with biological function.

Huang Li-Chieh LC Pan Xiaoyan X Yang Hongbing H Wan Lai Kin Derek LK Stewart-Jones Guillaume G Dorrell Lucy L Ogg Graham G

Scientific reports 20131023

Although peptides are well recognised biological molecules in vivo, their selection from libraries is challenging because of relative low affinity whilst in linear conformation. We hypothesized that multiplexed peptides and DNA on the surface of beads would provide a platform for enhanced avidity and the selection of relevant peptides from a library (ORBIT bead display). Using human immunodeficiency virus (HIV-1) gp120 as a target, we identify peptides that inhibit HIV-1 replication in vitro thr ...[more]

PMID: 24149829

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