An innovative tool for moving malaria PCR detection of parasite reservoir into the field.

ABSTRACT:

Background

To achieve the goal of malaria elimination in low transmission areas such as in Cambodia, new, inexpensive, high-throughput diagnostic tools for identifying very low parasite densities in asymptomatic carriers are required. This will enable a switch from passive to active malaria case detection in the field.

Methods

DNA extraction and real-time PCR assays were implemented in an "in-house" designed mobile laboratory allowing implementation of a robust, sensitive and rapid malaria diagnostic strategy in the field. This tool was employed in a survey organized in the context of the MalaResT project (NCT01663831).

Results

The real-time PCR screening and species identification assays were performed in the mobile laboratory between October and November 2012, in Ra

SUBMITTER: Canier L 

PROVIDER: S-EPMC3829804 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature