Efficient generation of functional epithelial and epidermal cells from human pluripotent stem cells under defined conditions.

ABSTRACT: Human pluripotent stem cells (hPSCs) have an unparalleled potential to generate limitless quantities of any somatic cell type. However, current methods for producing populations of various somatic cell types from hPSCs are generally not standardized and typically incorporate undefined cell culture components often resulting in variable differentiation efficiencies and poor reproducibility. To address this, we have developed a defined approach for generating epithelial progenitor and epidermal cells from hPSCs. In doing so, we have identified an optimal starting cell density to maximize yield and maintain high purity of K18+/p63+ simple epithelial progenitors. In addition, we have shown that the use of synthetic, defined substrates in lieu of Matrigel and gelatin can successfully facilitate

SUBMITTER: Selekman JA 

PROVIDER: S-EPMC3833387 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature