Molecular characterisation of virus in the brains of patients with measles inclusion body encephalitis (MIBE).

ABSTRACT:

Background

During 2009/10 a major measles epidemic caused by genotype B3 occurred in South Africa. Measles inclusion body encephalitis (MIBE) was diagnosed in a number of highly immuno-compromised HIV patients. The diagnosis was based on typical clinical and MRI findings and positive measles virus PCR in brain or CSF.To characterize the brain virus, nucleoprotein, matrix, fusion and haemagglutinin genes from 4 cases was compared with virus from acutely infected patients.

Methods

cDNA was synthesized using random primers and viral genes were amplified by nested RT-PCR. PCR products were sequenced in the forward and reverse direction and a contig of each gene was created. Sequences were aligned with reference sequences from GenBank and other local sequences.

Results

Bra

SUBMITTER: Hardie DR 

PROVIDER: S-EPMC3847183 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature