Signal transduction molecule patterns indicating potential glioblastoma therapy approaches.

ABSTRACT:

Purpose

The expression of an array of signaling molecules, along with the assessment of real-time cell proliferation, has been performed in U87 glioma cell line and in patients' glioblastoma established cell cultures in order to provide a better understanding of cellular and molecular events involved in glioblastoma pathogenesis. Experimental therapy was performed using a phosphatidylinositol-3'-kinase (PI3K) inhibitor.

Patients and methods

xMAP technology was employed to assess expression levels of several signal transduction molecules and real-time xCELLigence platform for cell behavior.

Results

PI3K inhibition induced the most significant effects on global signaling pathways in patient-derived cell cultures, especially on members of the mitogen-activated protein-ki

SUBMITTER: Cruceru ML 

PROVIDER: S-EPMC3848931 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature