Cervical cancer cells with positive Sox2 expression exhibit the properties of cancer stem cells.

ABSTRACT:

Background

Although Sox2 expression has been found in several types of cancer, it has not yet been used to identify or isolate CSCs in somatic carcinoma.

Methods

SiHa and C33A cells stably transfected with a plasmid containing human Sox2 transcriptional elements driving the enhanced green fluorescent protein (EGFP) reporter were sorted into the Sox2-positive and the Sox2-negative populations by FACS, and Sox2 expression was detected by western blot and immunohistochemistry. The differentiation, self-renewal and tumor formation abilities, as well as the expression of the stemness and the EMT related genes of the Sox2-positive and the Sox2-negative cervical cancer cells were characterized in vitro and in vivo.

Results

A pSox2/EGFP system was used to separate the Sox2-po

SUBMITTER: Liu XF 

PROVIDER: S-EPMC3904967 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature