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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

ABSTRACT: Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC3909901 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature

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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

Zhao Yicheng Y Dai Zhen Z Liang Yang Y Yin Ming M Ma Kuiying K He Mei M Ouyang Hongsheng H Teng Chun-Bo CB

Scientific reports 20140203

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells. ...[more]

PMID: 24487629

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