Informatics-based, highly accurate, noninvasive prenatal paternity testing.

ABSTRACT:

Purpose

The aim of the study was to evaluate the diagnostic accuracy of an informatics-based, noninvasive, prenatal paternity test using array-based single-nucleotide polymorphism measurements of cell-free DNA isolated from maternal plasma.

Methods

Blood samples were taken from 21 adult pregnant women (with gestational ages between 6 and 21 weeks), and a genetic sample was taken from the corresponding biological fathers. Paternity was confirmed by genetic testing of the infant, products of conception, control of fertilization, and/or preimplantation genetic diagnosis during in vitro fertilization. Parental DNA samples and maternal plasma cell-free DNA were amplified and analyzed using a HumanCytoSNP-12 array. An informatics-based method measured single-nucleotide polymorphis

SUBMITTER: Ryan A 

PROVIDER: S-EPMC3910294 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature