High-throughput screening of a Corynebacterium glutamicum mutant library on genomic and metabolic level.

ABSTRACT: Due to impressive achievements in genomic research, the number of genome sequences has risen quickly, followed by an increasing number of genes with unknown or hypothetical function. This strongly calls for development of high-throughput methods in the fields of transcriptomics, proteomics and metabolomics. Of these platforms, metabolic profiling has the strongest correlation with the phenotype. We previously published a high-throughput metabolic profiling method for C. glutamicum as well as the automatic GC/MS processing software MetaboliteDetector. Here, we added a high-throughput transposon insertion determination for our C. glutamicum mutant library. The combination of these methods allows the parallel analysis of genotype/phenotype correlations for a large number of mutants. In a pilo

SUBMITTER: Reimer LC 

PROVIDER: S-EPMC3913579 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature