Isotopic ratio outlier analysis global metabolomics of Caenorhabditis elegans.

ABSTRACT: We demonstrate the global metabolic analysis of Caenorhabditis elegans stress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5% (13)C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis. This labeling strategy provides several advantages over conventional approaches: (1) compounds arising from biosynthesis are easily distinguished from artifacts, (2) errors from sample extraction and preparation are minimized because the control and experiment are combined into a single sample, (3) measurement of both the molecular weight and the exact number of carbon atoms in each molecule provides extr

SUBMITTER: Stupp GS 

PROVIDER: S-EPMC3921967 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature