Large-scale mapping of transposable element insertion sites using digital encoding of sample identity.
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ABSTRACT:
SUBMITTER: Gohl DM
PROVIDER: S-EPMC3948795 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Publications
Large-scale mapping of transposable element insertion sites using digital encoding of sample identity.
Gohl Daryl M DM Freifeld Limor L Silies Marion M Hwa Jennifer J JJ Horowitz Mark M Clandinin Thomas R TR
Genetics 20131227 3
Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools. ...[more]
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