Dataset Information

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.

ABSTRACT:

Background

Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.

Materials and methods

After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl1 blue cells and DNA sequences were compared with Mo1,2 gene in M. oleifera.

Results

We obtained 3 PCR products (approximately 200, 300, and 400 bps).

Conclusion

After comparison of the sequences of 300bp band obtained from M. peregrina with Mo1,2 gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity.

SUBMITTER: Ghodsi R

PROVIDER: S-EPMC3950800 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.

Ghodsi Reihaneh R Sadeghi Hamid Mirmohammad HM Asghari Gholamreza G Torabi Sepideh S

Advanced biomedical research 20140127

<h4>Background</h4>Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.<h4>Materials and methods</h4>After preparation of M. peregrina callus, mRNA was extracted from t ...[more]

PMID: 24627865

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Dataset Information

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.

Background

Materials and methods

Results

Conclusion

Publications

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.

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