Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl1 blue cells.

ABSTRACT:

Background

Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina.

Materials and methods

After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl1 blue cells and DNA sequences were compared with Mo1,2 gene i

SUBMITTER: Ghodsi R 

PROVIDER: S-EPMC3950800 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature