High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis.

ABSTRACT: N(6)-methyladenosine (m(6)A) is the most ubiquitous mRNA base modification, but little is known about its precise location, temporal dynamics, and regulation. Here, we generated genomic maps of m(6)A sites in meiotic yeast transcripts at nearly single-nucleotide resolution, identifying 1,308 putatively methylated sites within 1,183 transcripts. We validated eight out of eight methylation sites in different genes with direct genetic analysis, demonstrated that methylated sites are significantly conserved in a related species, and built a model that predicts methylated sites directly from sequence. Sites vary in their methylation profiles along a dense meiotic time course and are regulated both locally, via predictable methylatability of each site, and globally, through the core meiotic circ

SUBMITTER: Schwartz S 

PROVIDER: S-EPMC3956118 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature