Monolayer spontaneous curvature of raft-forming membrane lipids.

ABSTRACT: Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H_II). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a H_II forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J₀ values are closer to zero. Interestingly, a significant positive J₀ value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J₀(<

SUBMITTER: Kollmitzer B 

PROVIDER: S-EPMC3963256 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature