The effect of cell subset isolation method on gene expression in leukocytes.

ABSTRACT: Multiple scientific disciplines require the isolation of specific subsets of blood cells from patient samples for gene expression analysis by microarray or RNA-sequencing, preserving disease- or treatment-related signatures. However, little is known with respect to the impact of different cell isolation methods on gene expression and the effects of positive selection, negative selection, and fluorescence activated cell sorting (FACS) have not previously been assessed in parallel. To address this knowledge gap, CD4+ T cells, CD8+ T cells, B cells, and monocytes were isolated from blood samples from five independent donors using positive immunomagnetic selection, negative immunomagnetic selection, and FACS. We hypothesized that positive selection and FACS would yield higher purity but may ha

SUBMITTER: Beliakova-Bethell N 

PROVIDER: S-EPMC3975050 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature