Combinatorial approach for large-scale identification of linked peptides from tandem mass spectrometry spectra.

ABSTRACT: The combination of chemical cross-linking and mass spectrometry has recently been shown to constitute a powerful tool for studying protein-protein interactions and elucidating the structure of large protein complexes. However, computational methods for interpreting the complex MS/MS spectra from linked peptides are still in their infancy, making the high-throughput application of this approach largely impractical. Because of the lack of large annotated datasets, most current approaches do not capture the specific fragmentation patterns of linked peptides and therefore are not optimal for the identification of cross-linked peptides. Here we propose a generic approach to address this problem and demonstrate it using disulfide-bridged peptide libraries to (i) efficiently generate large mass s

SUBMITTER: Wang J 

PROVIDER: S-EPMC3977190 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature