De novo transcriptome hybrid assembly and validation in the European earwig (Dermaptera, Forficula auricularia).

ABSTRACT:

Background

The European earwig (Forficula auricularia) is an established system for studies of sexual selection, social interactions and the evolution of parental care. Despite its scientific interest, little knowledge exists about the species at the genomic level, limiting the scope of molecular studies and expression analyses of genes of interest. To overcome these limitations, we sequenced and validated the transcriptome of the European earwig.

Methodology and principal findings

To obtain a comprehensive transcriptome, we sequenced mRNA from various tissues and developmental stages of female and male earwigs using Roche 454 pyrosequencing and Illumina HiSeq. The reads were de novo assembled independently and screened for possible microbial contamination and repeated eleme

SUBMITTER: Roulin AC 

PROVIDER: S-EPMC3983118 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature