Withagulatin A inhibits hepatic stellate cell viability and procollagen I production through Akt and Smad signaling pathways.

ABSTRACT:

Aim

To investigate the effects of the natural product Withagulatin A on hepatic stellate cell (HSC) viability and type I procollagen production. The potential mechanism underlying the pharmacological actions was also explored.

Methods

The effect of Withagulatin A on cell viability was evaluated in HSC and LX-2 cells using a sulforhodamine B (SRB) assay. Cell cycle distribution was analyzed using flow cytometry. Type I procollagen gene expression was determined using real-time PCR. Regulation of signaling molecules by Withagulatin A was detected using Western blotting.

Results

Primary rat HSCs and the human hepatic stellate cell line LX-2 treated with Withagulatin A (0.625-20 micromol/L) underwent a dose-dependent decrease in cell viability, which was associated with S

SUBMITTER: Liu Q 

PROVIDER: S-EPMC4007817 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature