Dataset Information
L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.
Ontology highlight
ABSTRACT: Background
L-alanine, acting through the GerA receptor, was recently found to be an efficient germinant in Bacillus licheniformis ATCC14580/DSM13.Results
In this study, we show that several of 46 examined B. licheniformis strains germinate remarkably slower than the type strain when exposed to L-alanine. These strains are not necessarily closely related, as determined by MLST (multi-locus sequence typing). Three of the slow-germinating strains were further examined in order to see whether nucleotide substitutions in the gerA sequences were responsible for the slow L-alanine germination. This was performed by complementing the transformable type strain derivate MW3ΔgerAA with gerA variants from the three slow-germinating strains; NVH1032, NVH1112 and NVH800.Conclusions
A wide selection of B. licheniformis strains was evaluated for L-alanine-induced germination efficiency. Our results show that gerA substitutions could only partially explain why spores of some B. licheniformis strains responded slower than others in the presence of L-alanine.
SUBMITTER: Madslien EH
PROVIDER: S-EPMC4021175 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
L-alanine-induced germination in Bacillus licheniformis -the impact of native gerA sequences.
BMC microbiology 20140422
<h4>Background</h4>L-alanine, acting through the GerA receptor, was recently found to be an efficient germinant in Bacillus licheniformis ATCC14580/DSM13.<h4>Results</h4>In this study, we show that several of 46 examined B. licheniformis strains germinate remarkably slower than the type strain when exposed to L-alanine. These strains are not necessarily closely related, as determined by MLST (multi-locus sequence typing). Three of the slow-germinating strains were further examined in order to se ...[more]