Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing.

ABSTRACT: Single-cell sequencing is a powerful tool for delineating clonal relationship and identifying key driver genes for personalized cancer management. Here we performed single-cell sequencing analysis of a case of colon cancer. Population genetics analyses identified two independent clones in tumor cell population. The major tumor clone harbored APC and TP53 mutations as early oncogenic events, whereas the minor clone contained preponderant CDC27 and PABPC1 mutations. The absence of APC and TP53 mutations in the minor clone supports that these two clones were derived from two cellular origins. Examination of somatic mutation allele frequency spectra of additional 21 whole-tissue exome-sequenced cases revealed the heterogeneity of clonal origins in colon cancer. Next, we identified a mutated ge

SUBMITTER: Yu C 

PROVIDER: S-EPMC4042168 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature