Semi-automatic quantification of neurite fasciculation in high-density neurite images by the neurite directional distribution analysis (NDDA).

ABSTRACT:

Background

Bundling of neurite extensions occur during nerve development and regeneration. Understanding the factors that drive neurite bundling is important for designing biomaterials for nerve regeneration toward the innervation target and preventing nociceptive collateral sprouting. High-density neuron cultures including dorsal root ganglia explants are employed for in vitro screening of biomaterials designed to control directional outgrowth. Although some semi-automated image processing methods exist for quantification of neurite outgrowth, methods to quantify axonal fasciculation in terms of direction of neurite outgrowth are lacking.

New method

This work presents a semi-automated program to analyze micrographs of high-density neurites; the program aims to quantify axon

SUBMITTER: Hopkins AM 

PROVIDER: S-EPMC4057284 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature