Functional dissection of sugar signals affecting gene expression in Arabidopsis thaliana.

ABSTRACT:

Background

Sugars modulate expression of hundreds of genes in plants. Previous studies on sugar signaling, using intact plants or plant tissues, were hampered by tissue heterogeneity, uneven sugar transport and/or inter-conversions of the applied sugars. This, in turn, could obscure the identity of a specific sugar that acts as a signal affecting expression of given gene in a given tissue or cell-type.

Methodology/principal findings

To bypass those biases, we have developed a novel biological system, based on stem-cell-like Arabidopsis suspension culture. The cells were grown in a hormone-free medium and were sustained on xylose as the only carbon source. Using functional genomics we have identified 290 sugar responsive genes, responding rapidly (within 1 h) and specifically

SUBMITTER: Kunz S 

PROVIDER: S-EPMC4065033 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature