Ontology highlight
ABSTRACT:
SUBMITTER: Leitner A
PROVIDER: S-EPMC4084482 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Leitner Alexander A Joachimiak Lukasz A LA Unverdorben Pia P Walzthoeni Thomas T Frydman Judith J Förster Friedrich F Aebersold Ruedi R
Proceedings of the National Academy of Sciences of the United States of America 20140617 26
The study of proteins and protein complexes using chemical cross-linking followed by the MS identification of the cross-linked peptides has found increasingly widespread use in recent years. Thus far, such analyses have used almost exclusively homobifunctional, amine-reactive cross-linking reagents. Here we report the development and application of an orthogonal cross-linking chemistry specific for carboxyl groups. Chemical cross-linking of acidic residues is achieved using homobifunctional dihy ...[more]