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Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry.


ABSTRACT: Mitochondrial protein interactions and complexes facilitate mitochondrial function. These complexes range from simple dimers to the respirasome supercomplex consisting of oxidative phosphorylation complexes I, III, and IV. To improve understanding of mitochondrial function, we used chemical cross-linking mass spectrometry to identify 2,427 cross-linked peptide pairs from 327 mitochondrial proteins in whole, respiring murine mitochondria. In situ interactions were observed in proteins throughout the electron transport chain membrane complexes, ATP synthase, and the mitochondrial contact site and cristae organizing system (MICOS) complex. Cross-linked sites showed excellent agreement with empirical protein structures and delivered complementary constraints for in silico protein docking. These data established direct physical evidence of the assembly of the complex I-III respirasome and enabled prediction of in situ interfacial regions of the complexes. Finally, we established a database and tools to harness the cross-linked interactions we observed as molecular probes, allowing quantification of conformation-dependent protein interfaces and dynamic protein complex assembly.

SUBMITTER: Schweppe DK 

PROVIDER: S-EPMC5321032 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry.

Schweppe Devin K DK   Chavez Juan D JD   Lee Chi Fung CF   Caudal Arianne A   Kruse Shane E SE   Stuppard Rudy R   Marcinek David J DJ   Shadel Gerald S GS   Tian Rong R   Bruce James E JE  

Proceedings of the National Academy of Sciences of the United States of America 20170127 7


Mitochondrial protein interactions and complexes facilitate mitochondrial function. These complexes range from simple dimers to the respirasome supercomplex consisting of oxidative phosphorylation complexes I, III, and IV. To improve understanding of mitochondrial function, we used chemical cross-linking mass spectrometry to identify 2,427 cross-linked peptide pairs from 327 mitochondrial proteins in whole, respiring murine mitochondria. In situ interactions were observed in proteins throughout  ...[more]

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