Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling.

ABSTRACT: Mitochondrial dysfunction is associated with many human diseases, including cancer and neurodegeneration, that are often linked to proteins and pathways that are not well-characterized. To begin defining the functions of such poorly characterized proteins, we used mass spectrometry to map the proteomes, lipidomes, and metabolomes of 174 yeast strains, each lacking a single gene related to mitochondrial biology. 144 of these genes have human homologs, 60 of which are associated with disease and 39 of which are uncharacterized. We present a multi-omic data analysis and visualization tool that we use to find covariance networks that can predict molecular functions, correlations between profiles of related gene deletions, gene-specific perturbations that reflect protein functions, and a global

SUBMITTER: Stefely JA 

PROVIDER: S-EPMC5101133 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature