Developing and mature human granulocytes express ELP 6 in the cytoplasm.

ABSTRACT:

Background

c3orf75 is a conserved open reading frame within the human genome and has recently been identified as the Elongator subunit, ELP6 [1]. The Elongator enzyme complex has diverse roles, including translational control, neuronal development, cell migration and tumorigenicity [2].

Objective

To identify genes expressed early in human eosinophil development.

Methods

Eosinophilopoiesis was investigated by gene profiling of IL-5 stimulated CD34+ cells; ELP6 mRNA is upregulated. A monoclonal antibody was raised to the recombinant protein predicted by the open reading frame.

Results

ELP6 transcripts are upregulated in a human tissue culture model of eosinophil development during gene profiling experiments. Transcripts are expressed in most tissue types, as show

SUBMITTER: Wagner LA 

PROVIDER: S-EPMC4085132 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature