Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting material.

ABSTRACT:

Aim

To demonstrate an inexpensive method for typing gastric cancer related single nucleotide polymorphisms (SNPs) using whole blood or paper-dried blood as starting materials.

Methods

PCR amplification is directly carried out from the whole blood or paper-dried blood sample without any DNA extraction step. Before PCR, a blood sample, four primers, and all of biological reagents necessary for PCR were added at a time; After PCR, the amplified products were directly separated by slab gel electrophoresis or microchip CE without any purification. SNP typing was performed by tetra-primer PCR with two inner primers specific to each allele and two outer primers defining the length of allele-specific amplicons. Genotypes were directly discriminated by the size of amplicons specific

SUBMITTER: Huang H 

PROVIDER: S-EPMC4087927 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature