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Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches.

ABSTRACT: Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution.

SUBMITTER: Izquierdo-Serra M

PROVIDER: S-EPMC4096865 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches.

Izquierdo-Serra Mercè M Gascón-Moya Marta M Hirtz Jan J JJ Pittolo Silvia S Poskanzer Kira E KE Ferrer Èric È Alibés Ramon R Busqué Félix F Yuste Rafael R Hernando Jordi J Gorostiza Pau P

Journal of the American Chemical Society 20140605 24

Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with ...[more]

PMID: 24857186

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