Identification of differentially methylated genes in the malignant transformation of ovarian endometriosis.

ABSTRACT:

Background

Key roles for epigenetic mechanisms in tumorigenesis are well accepted, while the relationship between gene methylation and malignant transformation of ovarian endometriosis (EMS) was seldom reported. In this study, we aimed to screen for aberrantly methylated genes associated with the malignant transformation of ovarian EMS and to preliminarily verify the reliability of screened results by detecting the methylation status and protein expression of the candidate gene in a larger scale of formaldehyde-fixed and paraffin-embedded (FFPE) samples.

Methods

Methylated CpG island amplification coupled with representational difference analysis (MCA-RDA) was performed on 3 couples of endometriosis-associated ovarian carcinoma (EAOC) fresh samples to identify differentially

SUBMITTER: Ren F 

PROVIDER: S-EPMC4105232 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature