The functional structure of central carbon metabolism in Pseudomonas putida KT2440.

ABSTRACT: What defines central carbon metabolism? The classic textbook scheme of central metabolism includes the Embden-Meyerhof-Parnas (EMP) pathway of glycolysis, the pentose phosphate pathway, and the citric acid cycle. The prevalence of this definition of central metabolism is, however, equivocal without experimental validation. We address this issue using a general experimental approach that combines the monitoring of transcriptional and metabolic flux changes between steady states on alternative carbon sources. This approach is investigated by using the model bacterium Pseudomonas putida with glucose, fructose, and benzoate as carbon sources. The catabolic reactions involved in the initial uptake and metabolism of these substrates are expected to show a correlated change in gene expressions an

SUBMITTER: Sudarsan S 

PROVIDER: S-EPMC4136102 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature